Promotion of differentiation of dendritic cells

Monocytes were isolated from the peripheral blood of healthy subjects and cultured in the presence of GM-CSF and IL-4 for 6 days, to prepare immature dendritic cells (iDC). On the 7th day, BioBran was added at various concentrations to the immature dendritic cells. The immature dendritic cells were incubated for 2 days and their maturation to dendritic cells was observed. The same operation was performed using two kinds of culture medium which have the effect of promoting the development of iDC to mature dendritic cells, designated mat DC1, and matDC2, respectively. In the immature dendritic cells (iDC), BioBran suppressed the expression of the monocyte marker CD14 and increased the expression of the dendritic cell marker CD83, both in a dose dependent manner. This result appears to show that BIoBran promotes the differentiation of dendritic cells.

Cholujova D., et al, “BioBran-augmented maturation of human monocyte-derived dendritic cells”, NEOPLASMA, 56, 2, 2009