BioBran in combination with a chemotherapeutic agent

Human breast cancer cells (MCF-7) (1 x 10⁴ cells) were seeded into the wells of a plate and incubated for 3 days in the presence of differing concentrations of BioBran, and the chemotherapeutic agent daunorubicin (DNR). Viable cells were counted by the MTT method, and the concentration of DNR which reduced the number of viable cancer cells by 50% (IC50) was determined. DNR inhibited the survival of MCF-7 cells in a concentration-dependent manner, with the IC50 being 1μM. When MCF-7 cells were cultured in the presence of BioBran and DNR, the IC50 of DNR for MCF-7 was reduced significantly (IC50: 0.2μM).

Ghoneum M. and S. Gollapudi, “MGN-3/Biobran, modified arabinoxylan from rice bran, sensitizes human breast cancer cells to chemotherapeutic agent, daunorubicin”, Cancer Detection and Prevention, 2008